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Glossary Abbreviations Used ACE affinity capillary electrophoresis AgNPs silver nanoparticles AMF anhydrous milk fat ATR attenuated total reflection AuNPs gold nanoparticles bLF bovine lactoferrin BOD biological oxygen demand bTF bovine transferrin CA carrier ampholyte CD circular dichroism CE capillary electrophoresis CGA chlorogenic acid cIEF capillary isoelectric focusing CM carboxymethyl CN casein COD chemical oxygen demand COVID-19 coronavirus disease 2019 DEAE diethylaminoethyl DHB 2,5-dihydroxybenzoic acid DLS dynamic light scattering DTPA diethylenetriaminepentaacetic acid DSC differential scanning calorimetry EDA ethylenediamine EDTA ethylenediaminetetraacetic acid ELISA enzyme-linked immunosorbent assay EOF electroosmotic flow ESI electrospray ionization Fe-NTA ferric nitrilotriacetate FTIR Fourier-transform infrared Fuc fucose Gal galactose GalNAc N -acetylgalactosamine GlcNAc N -acetylglucosamine GBM glioblastoma GPC gel permeation chromatography GPx glutathione peroxidase GR glutathione reductase GST glutathione- S -transferase HA hyaluronic acid HCV hepatitis C virus hLF human lactoferrin HMW high molecular weight HPLC high-performance liquid chromatography HSV herpes simplex virus hTF human transferrin HTST high temperatureshort time ICP-MS inductively coupled plasma mass spectrometry IEF isoelectric focusing IFI invasive fungal infections Ig immunoglobulins IL-1 interleukin 1 ITC isothermal titration calorimetry LAB lactic acid bacteria LC liquid chromatography LDH lactate dehydrogenase LF lactoferrin LFampin lactoferrampin LFcin lactoferricin LFR lactoferrin receptor LMCT ligandmetal charge transfer LPO lactoperoxidase LPS lipopolysaccharides MALDI-TOF/MS matrix-assisted laser desorption/ionization time of flight mass spectrometry Man mannose MD molecular docking MES 2-( N -morpholino)ethanesulfonic acid MF microfiltration MRM multiple reaction monitoring NEC necrotizing enterocolitis Nd:YAG neodymium-doped yttrium aluminum garnet NeuAc N -acetylneuraminic acid NF nanofiltration NF-B nuclear factor B pI isoelectric point PMF peptide mass fingerprint OA oleic acid PDB Protein Data Bank PEG polyethylene glycol PRDX peroxiredoxin PTT photothermal therapy PVA poly(vinyl alcohol) RO reverse osmosis ROS reactive oxygen species rTF rabbit transferrin SA serum albumin SARS-CoV-2 severe acute respiratory syndrome coronavirus 2 SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis SEC size-exclusion chromatography SERS surface enhanced Raman spectroscopy SOD1 superoxide dismutase 1 SP sulfopropyl TEM transmission electron microscopy TF transferrin TFA trifluoroacetic acid TFF tangential flow filtration TNF- tumor necrosis factor- UF ultrafiltration UHT ultrahigh temperature UVvis ultravioletvisible WPs whey proteins XRD X-ray diffraction 2D-PAGE two-dimensional polyacrylamide gel electrophoresis The research was financially supported in the frame of the project LIDER entitled Development of a preparative method for the isolation of biologically active lactoferrin, DPWP/LIDER-XIII/6/2023, financed by the National Centre for Research and Development

This happens when the lower esophageal sphincter (LES), the muscle that normally keeps acid in the stomach, relaxes or weakens

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