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The mitochondrial pellets (100200 g) were similarly extracted either in 0.6 M perchloric acid or 0.25 M potassium hydroxide in 50% ethanol and were diluted to 1:10 times for NAD + and 1:5 times for NADH in 100 mM sodium phosphate buffer

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Additionally, standard injection risks (infection at injection site, vein irritation for IV, etc.) apply, though proper sterile technique makes these complications uncommon

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