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The solution was passed through a column (Qiagen Inc., Hilden, Germany), to which 500 l of AW1 from DNeasy blood and tissue kit (Qiagen Inc., Hilden, Germany) was added, and the column was centrifuged at 6,000 g for 1 min

Three different models (linear, quadratic and logarithmic) were fitted to the data and their associated Akaike Information Criteria (AIC) were recorded in order to determine which model had the lowest AIC (i.e., provided a better fit to our data)

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