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This neural feedback stimulates efferent vagal fibers, which produce acetylcholine, which binds to 7 nicotinic acetylcholine receptors (7nAChR) on macrophages and immune cells, inhibiting pro-inflammatory cytokine production [120]

A total of 120 patients with infrainguinal arterial disease and Rutherford 56 foot gangrene were enrolled and divided into two groups: 60 received alprostadil post-IR, and 60 served as controls